Bacterial diversity in the rhizosphere of a transgenic versus a conventional maize (Zea mays)

L Vital-López, MA Cruz-Hernández, S Fernández-Dávila, A Mendoza-Herrera

Abstract


Genetically modified crops could cause negative effectson bacterial communities. In this study, we compared the bacterialcommunity structure of two maize cultivars to determine whetherthe transgenic cultivar exerts a negative effect on bacterial communitiesinhabiting the rhizosphere. Cultivars included the geneticallymodified maize (Zea mays), with the pat-gene conferring resistanceto the herbicide glufosinate (synonym: L-phosphinothricin), andthe hybrid, conventional maize. Metagenomic DNA was extractedfrom the rhizosphere of plants grown in a greenhouse. Single-strandconformation polymorphism, based on polymerase chain reactionamplifying a partial subunit rRNA gene was used to characterizeand generate genetic profiles that corresponded to the bacterial communitiesof the amplified products from the rhizosphere of the twomaize cultivars. Genetic profiles of the rhizospheres consisted ofdistinguishable profiles, based on the chosen primer pairs. Similarityanalyses of patterns found by binary matrix analyses showed nodifferences in the bacterial communities of the two cultivars. Thisanalysis showed that the microbial population’s structures of the conventionaland genetically modified maize were very homogeneous.Genetic modification did not adversely affect the structural bacterialcommunity in the rhizosphere of the transgenic maize cultivar.

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Los trabajos publicados en esta revista están bajo la licencia Creative Commons Atribución-NoComercial 2.5 Argentina.

Phyton. International Journal of Experimental Botany. ISSN print 0031-9457 ISSN en line 1851-5657 Fundación Rómulo Raggio Gaspar Campos 861,  Vicente López, Buenos Aires, Argentina (C.P. 1638) Tel 54 11 4791-0868 / 4796-1456 revistaphyton@fund-romuloraggio.org.ar www.revistaphyton.fund-romuloraggio.org.ar