Biochemical and molecular evidences of the presence of a plant natriuretic peptide in garlic (Allium sativum L.)

CM Valle-Rodríguez, S Valdés-Rodríguez, José Francisco Morales Domínguez, NL Vasco-Méndez, C Garcidueñas-Piña, RE Soria-Guerra

Abstract


The natriuretic peptides (NP) are vertebrate hormonesinvolved in the regulation of ion and water homeostasis in the cell.In plants, natriuretic peptides (PNP) have been identified by molecularand biochemical methods. It has been suggested that PNPs,have similar functions to the PN, since they mediate (1) ion transportthrough the cell, (2) the opening and closing of Na+ and K+ channelsthrough the stimulation and synthesis of a second messenger such ascGMP, and (3) the aperture of stomata. In this study, a PNPs genewas partially identified by PCR in garlic (Allium sativum L.), whichwas called AsPNP, and showed a similarity of 96% with the AtPNP-Aof Arabidopsis thaliana. The deduced amino acid sequence of AsPNPdisplayed four cysteine and the highly conserved reason (i.e., K [VI]VD) in PNPs. The modelling of AsPNP in amino acids showed athree-dimensional structure similar to AtPNP-A and to the domainI of expansins. The real-time PCR analysis identified at least two possiblecopies of this gene in garlic. The immunodetection with antibodiesdirected to human NPs receptors suggests the presence of similarreceptors in garlic leaves. It was also found that a protein fractionpurified by ion exchange chromatography from garlic leaves was ableto stimulate stomatal opening in cuticles of Opuntia ficus-indica. Theseevidences demonstrate the presence of NP in garlic plants.

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Phyton. International Journal of Experimental Botany. ISSN print 0031-9457 ISSN en line 1851-5657 Fundación Rómulo Raggio Gaspar Campos 861,  Vicente López, Buenos Aires, Argentina (C.P. 1638) Tel 54 11 4791-0868 / 4796-1456 revistaphyton@fund-romuloraggio.org.ar www.revistaphyton.fund-romuloraggio.org.ar