Cladistics of Mexican nopal genotypes (Opuntia spp.) based on AFLP markers and fruit characters

EA Espinoza-Sánchez, H Silos-Espino, S Flores-Benitez, LL Valera-Montero, E Rodríguez-Salazar, C Gallegos-Vázquez, F guevara-Lara, M González-Chavira, HS Guzmán-Maldonado

Abstract


Dendrograms were constructed based on AFLP markers and a table was used to group fruit characters of wild and cultivated genotypes of Mexican nopal (Opuntia spp.). The AFLP dendrogram obtained using ≈57.5 markers (from primers Mse I-CAC/Eco RI-AAG) did not agree with the known taxonomic classification for this species. Additionally, a complementary table for fruit characters (morphological and °Brix) showed a wide range for wild genotypes, e.g. from 21-22 °Brix for ‘Tapón Blanco’ and ‘Guilanche’ genotypes, to 5.0-8.2 °Brix for xoconostles (acidic fruits). Even though there were some divergences with taxonomic classification, it is expected that both genetic and physicochemical information would contribute to a more appropriate identification of Opuntia genotypes that, in turn, may be used in breeding programs of this genus.

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Phyton. International Journal of Experimental Botany. ISSN print 0031-9457 ISSN en line 1851-5657 Fundación Rómulo Raggio Gaspar Campos 861,  Vicente López, Buenos Aires, Argentina (C.P. 1638) Tel 54 11 4791-0868 / 4796-1456 revistaphyton@fund-romuloraggio.org.ar www.revistaphyton.fund-romuloraggio.org.ar