Relationship of Interleukin-6 G-174C and G-572C and Interleukin-1 C-511T and C+3953T Polymorphisms with Ischemic Heart Disease in Quito, Ecuador

Rosa Inés Colina Cifuentes, Luis Alejandro Cárdenas Argüello, Edwin Roberto Guzman Clavijo, Rommel Oswaldo Espinoza de los Moneteros Duche, Fabian Santiago Cruz Murillo, Fátima Leticia Caiza Llumiquinga


Background: It has been recently found that inflammatory processes are directly related to the development of atheroscleroticplaque, causing ischemic heart disease. For this reason, every molecule related to these processes is critically important. Interleukins(IL) are proinflammatory cytokines, and their polymorphisms seem to increase the inflammatory progress. IL-1 and IL-6 polymorphismsare the ones most significantly associated with ischemic heart disease.Objectives: The aim of this study was to establish the relationship of IL-6 G-174C and G-572 C and IL-1 C-511T and C+3953Tpolymorphisms with ischemic heart disease.Methods: A retrospective study of 76 cases and 76 controls was carried out in patients attending the hemodynamics service of CarlosAndrade Marín Hospital (HCAM) of Quito, Ecuador. Genotyping was done on the basis of polymerase chain reaction with restrictionenzymes (PCR-RFLP).Results: Among the four polymorphisms studied, only IL-6 -174 GG was significantly associated with ischemic heart disease. Thelogistic regression analysis used to determine the most important predictors of ischemic heart disease showed that the IL-6 -174 GGgenotype was associated with an increased risk of independently presenting ischemic heart disease (OR 4.065, p ≤0.001).Conclusions: The GG genotype of IL-6 G-174C polymorphism confers a fourfold higher risk of developing ischemic heart disease,while the remaining three polymorphisms do not pose risks in this human population.


Tarragó Amaya E, Soca PE, Cruz Lage LA, Lozano YS. Factores de riesgo y prevención de la cardiopatía isquémica. CCM 2012;16(2).

García-Moll X, Kaski JC. Cardiopatía isquémica: marcadores de inflamación y riesgo cardiovascular. Rev Esp Cardiol 2015;52:990- 1003.

Navarro-López F. Bases genéticas de la enfermedad coronaria. Rev Esp Cardiol 2002;55:413-31.

Schargrodsky H, Hernández-Hernández R, Champagne BM, Silva H, Vinueza R, Silva Ayçaguer LC, et al; CARMELA Study Investiga¬tors. CARMELA: assessment of cardiovascular risk in seven Latin American cities. Am J Med 2008;121:58-65.

Vohnout B, Castelnuovo A, Trotta R, D’orazio A, Panniteri G, Montali A, et al. Interleukin-1 gene cluster polymorphisms and risk of coronary artery disease. Haematologica 2003;88:54-60.

Lima-Neto LG, Hirata RD, Luchessi AD, Silbiger VN, Stephano MA, Sampaio MF, et al. CD14 and IL6 polymorphisms are associated with a pro-atherogenic profile in young adults with acute myocardial infarc¬tion. J Thromb Thrombolysis 2013;36:332-40.

Mendoza C. El Valor P en Epidemiología. Rev Chil Salud Publica 2006;10:47-51.

Rios DL, Cerqueira CC, Bonfim-Silva R, Araújo LJ, Pereira JF, Gadelha SR, et al. Interleukin-1 beta and interleukin-6 gene polymor¬phism associations with angiographically assessed coronary artery disease in Brazilians. Cytokine 2010;50:292-6.

Petrola CG, Naveda R, Chacón MR, Petrola I, Flores M, Pacheco M. Interleucina 6 en pacientes con cardiopatía isquémica / Serum levels of interleukin 6 in patients with a diagnosis of ischemic cardiopathy. Salus 2005;9:12-5.

Ozdemir O, Gundogdu F, Karakelleoglu S, Sevimli S, Pirim I, Acikel,M, et al. (2008). Comparison of serum levels of inflammatory markers and allelic variant of interleukin-6 in patients with acute coronary syndrome and stable angina pectoris. Coron Artery Dis 2008;19:15-19.

Deliargyris E, Raymond R, Theoharides T, Boucherb W, Tate D, Dehmer G. Sites of interleukin-6 release in patients with acute coronary syndromes and in patients with congestive heart failure. Am J Cardiol 2000;86:913-8.

Fishman GI, Chugh SS, Dimarco JP, Albert CM, Anderson ME, Bonow RO, et al. Sudden cardiac death prediction and prevention: report from a National Heart, Lung, and Blood Institute and Heart Rhythm Society Workshop. Circulation 2010;122:2335-48.

Sudomoina MA, Sukhinina TS, Barsova RM, Favorov AV, Shakhnovich RM, Titov BV, et al. Complex analsis of association of inflammation genes with myocardial infarction. Mol Biol (Mosk) 2010;44:463-71.

Kofler S, Nickel T, Weis M. Role of cytokines in cardiovascular diseases: a focus on endothelial responses to inflammation. Clin Sci (Lond) 2005;108:205-13.

Giacconi R, Cipriano C, Albanese F, Boccoli G, Saba V, Olivieri F, et al. The -174G/C polymorphism of IL-6 is useful to screen old subjects at risk for atherosclerosis or to reach successful ageing. Exp Gerontol 2004;39:621-8.

Nauck M, Winkelmann BR, Hoffmann MM, Böhm BO, Wieland H, März W. The interleukin-6 G (-174)C promoter polymorphism in the LURIC cohort: no association with plasma interleukin-6, coronary artery disease, and myocardial infarction. J Mol Med (Berl) 2002;80:507-13.

Georges JL1, Loukaci V, Poirier O, Evans A, Luc G, Arveiler D, et al. Interleukin-6 gene polymorphisms and susceptibility to myocar¬dial infarction: the ECTIM study. Etude Cas-Témoin de l’Infarctus du Myocarde. J Mol Med (Berl) 2001;79:300-5.

Humphries SE, Luong LA, Ogg MS, Hawe E, Miller GJ. The interleukin-6 -174 G/C promoter polymorphism is associated with risk of coronary heart disease and systolic blood pressure in healthy men. Eur Heart J 2001;22:2243-52.

Libra M, Signorelli SS, Bevelacqua Y, Navolanic PM, Bevelacqua V, Polesel J, et al. Analysis of G(-174)C IL-6 polymorphism and plasma concentrations of inflammatory markers in patients with type 2 dia¬betes and peripheral arterial disease. J Clin Pathol 2006;59:211-5.

Full Text


  • There are currently no refbacks.

Licencia Creative Commons
The documents published in this journal are under the licencia Creative Commons Atribución-NoComercial-Compartir-Igual 2.5 Argentina.

Revista argentina de cardiología. ISSN en línea 1850-3748. Argentine journal of cardiology (English ed. Online ISSN 2314-2286) Sociedad Argentina de Cardiología. Azcuénaga 980 (C1115AAD),Ciudad Autónoma de Buenos Aires, República Argentina. Tel. (54 11) 4961-6027/8/9 Fax: 4961-6020